Research project
Objective 1
Global survey
Reveal ARG profiles and bacterial diversity associated with urban, rural and agricultural areas across Canada
using a long-term sampling method (vehicles AC filters). Samples analysis using qPCR and genome sequencing.
ARG identification using AI.
Objective 2
Air sampling
Determine bacterial diversity and ARG profile in bioaerosols during specific urban, rural and agricultural activities by using high-volume air samplers.
Objective 4
ARG long distance transport
Melting glaciers and permafrost can release microbes that are potentially thousands of years old, representing another potential source of antibiotic-resistant genes (AGRs). Collecting air from samples in polar desert sites. GRA detection by qPCR and genome sequencing.
Objective 5
Integrated Assessment Model on Antimicrobial Resistance- role of bioaerosols
Canadian integrated assessment model for ARG framework will be expanded to include dissemination of AMR through aerosols adding to environmental pathways
addressing dissemination through water, manure application to crops and sewage.